|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
09/11/2017 |
Actualizado : |
12/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
DOI : |
10.5539/sar.v6n3p |
Idioma : |
Inglés |
Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
|
Marc : |
LEADER 02541naa a2200289 a 4500 001 1057747 005 2018-12-12 008 2017 bl uuuu u00u1 u #d 022 $a1927-050X (Print) // 1927-0518 (Online) 024 7 $a10.5539/sar.v6n3p$2DOI 100 1 $aFEDERICI, M. 245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. 520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education 653 $aDGGE 653 $aMETAGENOMICS 653 $aPYROSEQUENCING 653 $aRHIZOSPHERE 653 $aSOIL BACTERIA 653 $aTRANSGENIC MAIZE 700 1 $aBAJSA, N. 700 1 $aLAGURARA, P. 700 1 $aREVALE, S. 700 1 $aMARCONDES, J.A. 700 1 $aDALLA RIZZA, M. 773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
18/10/2019 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
QUINTANS, G.; BANCHERO, G.; CARRIQUIRY, M.; LÓPEZ, C.; BALDI, F. |
Afiliación : |
GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANA CARRIQUIRY; CARLOS LÓPEZ; FERNANDO SEBASTIAN BALDI REY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Efecto de la condición corporal y la restricción del amamantamiento con y sin presencia del ternero sobre la producción de leche, anestro posparto y crecimiento de los terneros |
Complemento del título : |
Capítulo 3. Alternativas de manejo nutricional, control del amamantamiento y tratamientos hormonales durante el posparto. |
Fecha de publicación : |
2008 |
Fuente / Imprenta : |
In: QUINTANS, G.; VELAZCO, J.I.; ROIG, G. (Eds.). Seminario de actualización técnica: cría vacuna. Montevideo (Uruguay): INIA, 2008. |
Páginas : |
p. 172-181 |
Serie : |
(INIA Serie Técnica ; 174) |
ISBN : |
978-9974-38-251-0 |
ISSN : |
1688-9266 |
Idioma : |
Español |
Thesagro : |
BOVINOS; BOVINOS DE CARNE; CONDICION CORPORAL; DESTETE; GANADERIA; GANADO DE CARNE; NUTRICION; NUTRICION ANIMAL; REPRODUCCIÓN; TERNERO; URUGUAY. |
Asunto categoría : |
-- A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7754/1/st-174-2008-p.172-181.pdf
|
Marc : |
LEADER 01076naa a2200337 a 4500 001 1008131 005 2019-10-18 008 2008 bl uuuu u00u1 u #d 020 $a978-9974-38-251-0 022 $a1688-9266 100 1 $aQUINTANS, G. 245 $aEfecto de la condición corporal y la restricción del amamantamiento con y sin presencia del ternero sobre la producción de leche, anestro posparto y crecimiento de los terneros 260 $c2008 300 $ap. 172-181 490 $a(INIA Serie Técnica ; 174) 650 $aBOVINOS 650 $aBOVINOS DE CARNE 650 $aCONDICION CORPORAL 650 $aDESTETE 650 $aGANADERIA 650 $aGANADO DE CARNE 650 $aNUTRICION 650 $aNUTRICION ANIMAL 650 $aREPRODUCCIÓN 650 $aTERNERO 650 $aURUGUAY 700 1 $aBANCHERO, G. 700 1 $aCARRIQUIRY, M. 700 1 $aLÓPEZ, C. 700 1 $aBALDI, F. 773 $tIn: QUINTANS, G.; VELAZCO, J.I.; ROIG, G. (Eds.). Seminario de actualización técnica: cría vacuna. Montevideo (Uruguay): INIA, 2008.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|